Journal: Materials Today Bio
Article Title: Dorsal root ganglion-targeted analgesic delivery for effective relief of neuropathic pain
doi: 10.1016/j.mtbio.2025.102025
Figure Lengend Snippet: In vivo DRG-targeting ability of BK-LNPs in SNI and CINP. (A) t-distributed stochastic neighbor embedding (t-SNE) visualization of Adam8 mRNA expression patterns across DRG cellular subpopulations and (B) RNAscope imaging for Adam8 mRNA in the DRG neurons following SNI modeling. (C–D) Relative Adam8 mRNA expression level in the DRG on the 7th and 28th days following SNI modeling. (E) CLSM images of the bilateral DRGs ipsilateral and contralateral to SNI surgical site and (F) corresponding quantitative analysis of RhB fluorescence. Tuj1 was a neuronal marker. (G) Schematic illustration for the bilateral DRGs ipsilateral and contralateral to SNI surgical site. (H) Relative Adam8 mRNA expression level in the DRG as well as in the heart, liver, spleen, lung, and kidney on the 7th day following CINP modeling. (I) CLSM images of the DRG in CINP and (J) corresponding quantitative analysis of RhB fluorescence. For CLSM imaging, mice were intravenously injected with LNP/RhB and BK-LNPs/RhB and at 6 h post-injection, mice were euthanized for analysis. Data were expressed as mean ± SEM (n = 3 for C and D; n = 5 for F, H, and J). ∗∗ p < 0.01 and ∗∗∗ p < 0.001.
Article Snippet: The obtained DRG sections (14 μm in thickness) were blocked with 10 % BSA for 1 h and then sequentially incubated with neuronal class III β-tubulin (Tuj1) primary antibody (D71G9, rabbit, Cell Signaling Technology, #5568, 1:1000) overnight and its fluorescence-labeled secondary antibody (Alexa Fluor 488 anti-rabbit IgG, donkey, ThermoFisher, #A-21206, 1:500) for 2 h. The fluorescence of Tuj1 and RhB was captured on the SP8 CLSM using the corresponding filters to evaluate the targeting ability of BK-1361.
Techniques: In Vivo, Expressing, RNAscope, Imaging, Fluorescence, Marker, Injection
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